What is that graph with peaks on a COA?

Updated: 2026-09-09 · Buying, quality and COA

Short answer

It is a chromatogram: a record of how a sample's components separate as they pass through a column. Each peak is something that came out at a given time, and its area is used to calculate proportions. It shows separation, not identity: on its own it does not confirm which molecule each peak is.

In plain English

  • The horizontal axis is retention time; the vertical axis is detector signal.
  • Purity percentage is usually main peak area divided by total peak area.
  • Identity is confirmed by other testing, typically mass spectrometry.
  • A chromatogram with no scales, no method and no lot identification tells you little.

What we know

  • HPLC and mass spectrometry are complementary tests with different purposes.
  • Serious analytical reports include method, conditions, date and lot.

What we don't know yet

  • A standalone image of a chromatogram cannot be traced to its source.
  • Anything eluting outside the analysis window never appears on the graph.

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